Genetic Variation and Clone Diversity of an Invasive Species Eupatorium Adenophorum

Abstract: The relationship between existing vegetation diversity and its pollen diversity can be used to infer past vegetation diversity from the spectra of fossil pollen. A better understanding of the pollen dispersing in surface soil under extant vegetation is necessary to the interpretation of fossil pollen spectra for deriving the information on past vegetation. We developed a new approach to identification of Chenopodiaceous pollens in surface soils to the species level while current palynological methods have less ability to do so even at a genus level. The surface soil samples for pollen analyses were collected in the central area of Junggar Desert Plains, Xinjiang, China. Fresh leaves of 19 Chenopodiaceous species were collected for DNA sequencing. A database of Chenopodiacious internal transcribed spacer regions (ITS1) of nucleic ribosomal DNA was then established. The genomic DNA of a Chenopodiaceae pollen grain was extracted, and the ITS1 region of pollen grain DNA was amplified using a nested polymerase chain reaction and then sequenced. By comparing ITS1 sequences of the samples to a reference database, a single chenopodiaceous surface soil pollen grain was identified to a species level. This method makes it possible for interpreting surface pollen spectra to the species level and has a significant potential for enhancing the ability to interpret fossil pollen spectra in paleoecological and paleoclimatic studies for describing paleovegetation for its reconstruction of interest to modern botanists and ecologists.Crofton weed (Eupatorium adenophorum) is an invasive species which is widely distributed in Southwest of China. The Layering propagation strategy was usually omitted by the researchers in spite of it is helpful for invasion as well as apomictic strategy. AFLP marker was used to analysis genetic variation and clone diversity of crofton weed to find out what makes the invasive species with such mating system so competitive. Individuals of 17 populations including one population of Burma wer…
Key words: Chenopodiacea; Single pollen identification; Incompatibility analysis; Invasive ecology; Genetic variation; AFLP

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