Abstract: [ Objective ] In order to investigate the clinical significance and the mechanism of hepatocellular carcinoma (HCC) of hepatitis B virus basic core promoter (HBV BCP) mutation in patients with chronic hepatitis B virus infection. [Methods] (1) project subject: 176 patients (Chronic hepatitis B with mild , moderate and sever; liver cirrhosis, Chronic fulminant hepatitis and HCC ) with chronic hepatitis B virus infection were studied. (2) project methods: ① The A to T mutation at nucleotide 1762 and G to A mutation at nucleotide 1764 were determined by the method of polymerase chain reaction (PCR) microplate hybridization ELISA in these patients. ② The serum cytokines (IL-10、 IL-12、 TNF-α and IFN-γ) of these patients were measured by specific-ELISA. ③ Serum HBV DNA of these patients were detected by fluorescence PCR. ④ Serum HBV markers were determined by ELISA.⑤ Th…
Key words: Hepatitis B virus; Polymerase chain reaction; basic core promoter; gene mutation; interleukin 10; interleukin 12; tumor necrosis factorα; interferonγ
A Study of the Clinical Significance and the Mechanism of Hepatocellular Carcinoma (HCC) of Hepatitis B Virus Basic Core Promoter (HBV BCP) Mutation in Patents with Chronic Hepatitis B Virus Infection
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