The Effect of Anisodamine on Cardiac Arrest Rat and Hypoxia/Reoxygenation Cardiomyocyte

Abstract: According to some researches in CPR,the increase of dose of epinephrine could increase cardiac and cerebral perfusion pressure and blood perfusion flow,but it could also do damage to the myocardia and the brain tissues,resulting in the decrease success rate of CPR.anisodamine can increase the coronary blood flow,improve the microcirculation,reduce the aggregation of platelet,prevent the thrombosis,and alleviate the ischemia-reperfusion injury of the myocardia and the brain tissues,etc.In the early stage of CPR the application of anisodamine may improve tissue perfusion, improve the function of cardiac and cerebral tissues,reduce the side-effect of epinephrine,increase the CPR success rate and improve the prognosis.Therefore,the function of anisodamine in CPR was experimented and studied.Objective To establish a standard rats’ cardiac arrest and CPR model for preclinical medicine research,using blind method to deliver the medicine,observe anisodamine’s effect on functions of myocardia and brain tissues of rats which suffers from cardiac arrest during CPR,and further observe anisodamine’s protection effect on hypoxia/reoxygenation cardiomyocyte in terms of apoptosis.Methods There were four parts in the experiment.In part